A lab-scale sequencing batch reactor (SBR) was built and used for the treatment of synthetic wastewater to investigate the activity and distribution of hydrolytic enzymes (α-amylase, α
Principal investigator James A. Nicell, PhD, PEng (Professor) Department of Civil Engineering and Applied Mechanics McGill University Background In an attempt to overcome some of the problems associated with traditional chemical and biological waste treatment systems, recent research has focused on the environmental applications of pure enzymes that have been isolated from their parent organisms.
Get PriceThe biological treatment of a synthetic dairy wastewater containing high levels of oil and grease (200, 600 and 1000 mg/l) was investigated, using two identical UASB reactors. One reactor was fed with wastewater from an upstream enzymatic hydrolysis step and the other with raw wastewater.
Get PriceEnzyme activity is influenced by many factors, such as temperature, pH, and oxygen conditions; oxygen is one of the most important parameters in biological wastewater treatment. Oxygen conditions influenced microorganism community composition, growth characteristics, and metabolic pathways [ 17 , 18 ].
Get PriceBio-augmentation of waste treatment systems with enzymes and/or bacteria has been shown to: Improve treatment system efficiency. Reduce odor. Reduce sludge volume. Improve the clarity of ponds. Reduce COD (chemical oxygen demand) in the effluent. Improve effluent water quality more consistently.
Get PriceBased on this fundamental knowledge, and recent developments in enzyme immobilisation techniques, microbiological enzymic treatment strategies are proposed to formulate a new generation of biological wastewater treatment processes for the biodegradation of environmentally challenging OC compounds.
Get PriceStudying the regulation of related enzymes’ activity is essential to minimize N2O emissions during wastewater treatment. This paper aims to review the poorly understood related enzymes that most commonly involved in producing and consuming N2O in terms of their nature, structure and catalytic mechanisms.
Get Pricecrucial role in wastewater treatment applications. Enzymes can specifically act on and remove recalcitr ant p ollutants by precipitation a nd transformation to o ther pr oducts a nd
Get PriceBiological methods of wastewater treatment have received much attention in recent years. Their increasing importance is by virtue of the possibility of total oxidation and sanitation of many impurities, including toxic ones, while requiring comparatively low operating cost and simple equipments, through microorganisms or their metabolites
Get PriceHowever, high rates of pH/thermal enzyme denaturation and low rates of enzyme retention, recovery, and reusability challenge the contaminant removal efficiency and the economic feasibility of enzymatic wastewater treatment . Therefore, the stabilisation of the enzymes is necessary for practical implementation of enzymic treatment.
Get PriceExtracellular enzymatic activity of two hydrolases in wastewater treatment for biological nutrient removal Author: Berrio-Restrepo, JorgeMario, Saldarriaga, JulioCésar, Correa, MauricioAndrés, Aguirre, NéstorJaime Source: Applied microbiology and biotechnology 2017 v.101 no.19 pp. 7385-7396 ISSN: 0175-7598 Subject:
Get Pricefor operating the biological activity in wastewater treatment plants > ApplicAtion notE #3 - novEmbEr 2008 The technique of ATP-metry allows a fast measure of the concentration of the active biomass in water. This method is based on the quantification of the Adenosine TriPhosphate (ATP), which is an essential molecule in the microbial life.
Get PriceThe wastewater after primary, secondary biological, and tertiary treatments contains chemical surfactants at a considerable concentration (60 mg/L). In the present investigation, lipolytic micro-organisms that are capable of utilizing wetting agents (vegetable fatliquor) as the substrate were used to produce lipase.
Get PriceThis study is focused on biodegradation of micropollutants using different different enzymatic, photocatalytic and biological process. Various extracellular ligninolytic enzymes were selected and tested for degradation of pharmaceuticals in real wastewater. Ligninolytic enzymes have high degradation potential and been already applied for successful removal of various persistent organopollutants.
Get PriceHQ730879 with lipase activity (0.3 U/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/L total fat content. The pretreatment was optimized for 48 h hydrolysis time, at 45æ% C with 10% v/v enzymatic extract. The biological treatment of synthetic dairy
Get PriceIn this study, a pilot wastewater treatment plant was used to evaluate the co-treatment of biological-staining residues and domestic wastewater under non-sterile conditions. A novel microbial consortia formed by Trametes versicolor, Trametes sp, Pleurotus ostreatus, Pseudomonas fluorescens, Pseudomonas azotoformans, Pseudomonas sp, Enterobacter xianfangensis and Bacillus subtillis was
Get PriceThe concentration of hydrogen ions in wastewater affects the enzyme activity. Each enzyme has maximal efficiency under an optimum pH otherwise there will be no enzymatic activity due to denaturation of enzymes. Therefore, in this study influence of pH on the colour and COD removal efficiencies was examined by varying its range (5, 7 and 9) and
Get PriceThe biological treatment of wastewater is characterized by groups of microorganisms and the enzymes secreted by them. These hydrolytic enzymes are vital for the rate-limiting step of hydrolysis in the treatment of extracellular polymeric substances. The present article reviews the enzymatic activity of activated sludge, promoting that the connection between the chemical composition of
Get PriceEnzymatic activity of activated sludge in biological treatment of wastewater containing dimethylamine, dimethylformamide and methylethylketone. Sztajer H, Lejczak B, Pawlaczyk-Szpilowa M. Activated sludge treating synthetic wastewater carrying dimethylamine (DMA), dimethylformamide (DMF) and methylethylketone (MEK) was examined.
Get Pricerepresentative of in situ activity of pollutant-degrading microorganisms. in recent years, the enzymology of biological remediation and practical applications of enzyme technologies in wastewater treatment has been receiving increased attention (13). the study of enzyme activities can provide suggestive information
Get PriceIn the present study, the interactive effects of temperature and cultured bacteria on the performance of a biological treatment system of wastewater from a local yeast producing plant were investigated. The main objective of this study was to optimize the operating parameters that reduce organic load and colour. Biological treatment was conducted using a Central Composite Design (CCD) and
Get PriceEnzymatic treatment, using the laccasemediator system, is a novel biochemical process that has been shown to effectively treat some PPCPs. This study investigates the efficacy of the laccase-mediator system to treat PPCPs using a process that can be easily implemented at an existing wastewater treatment plant.
Get PriceHQ730879 with lipase activity (0.3 U/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/L total fat content. The pretreatment was optimized for 48 h hydrolysis time, at 45æ% C with 10% v/v enzymatic extract. The biological treatment of synthetic dairy
Get PriceTrends in wastewater genomics. For decades, the putative key players in wastewater treatment processes were those that were culturable and well studied, while the really important species remained non‐culturable or unknown (Crocetti et al., 2000; Chouari et al., 2005a).A variety of genomes of cultured microbes have been sequenced in the past 5 years, but now the focus is shifting towards
Get Pricefor operating the biological activity in wastewater treatment plants > ApplicAtion notE #3 - novEmbEr 2008 The technique of ATP-metry allows a fast measure of the concentration of the active biomass in water. This method is based on the quantification of the Adenosine TriPhosphate (ATP), which is an essential molecule in the microbial life.
Get PriceExtracellular enzymatic activity of two hydrolases in wastewater treatment for biological nutrient removal Author: Berrio-Restrepo, JorgeMario, Saldarriaga, JulioCésar, Correa, MauricioAndrés, Aguirre, NéstorJaime Source: Applied microbiology and biotechnology 2017 v.101 no.19 pp. 7385-7396 ISSN: 0175-7598 Subject:
Get Price2.2.1. Optimization of cellulase activity Before commencing the enzymatic pretreatment studies, the optimization of the pH and the temperature for the maximum enzyme activity was carried out for the enzyme which was used in the study. The enzyme was assayed at different pH, ranging from 3.0–6.0, using citrate buffer (0.05 M).
Get PriceIn this study the enzymatic degradation of hormones and endocrine disrupting compounds (EDCs) was investigated in artificial mixtures and in real wastewater by fungal laccases (Trametes versicolor, Myceliophthora thermophila). Several studies have already reported the successful enzymatic degradation of EDCs. However, with regards to a large-scale application, the influence of some factors
Get Priceet al., 2008). However, these studies on vermifilter focused on treatment efficiency of wastewater, and few on earthworm population dynamics and enzymatic activity in the vermifiltration wastewater treatment process. Wastewater had likely led to an influence on earthworm population dynamics and enzymatic activity, because it
Get PriceWastewater treatment processes can be divided into three classes; physical, chemical and biological. Biological treatment processes, in turn, can be classified as either aerobic or anaero-bic. Each of these two classes of wastewater treatment has dis-tinct associated advantages and disadvantages (Table 1). From
Get PriceBiomonitoring in the control system of biological wastewater treatment in pulp and paper industry V Rudakova1, K Bolotova1 the control system of biological wastewater treatment of pulp and paper production pays great attention to biomonitoring.One of the most One of these methods is determination the enzymatic activity of active sludge
Get PriceBiological treatment is an important and integral part of any wastewater treatment plant that treats wastewater from either municipality or industry having soluble organic impurities or a mix of the two types of wastewater sources. The obvious economic advantage, both in terms of capital investment and
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