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Quantification of rat neurophysins by polyacrylamide gel electrophoresis (PAGE): application to the rat with hereditary hypothalamic diabetes
Quantification of rat neurophysins by polyacrylamide gel electrophoresis (PAGE): application to the rat with hereditary hypothalamic diabetes insipidus Ann N Y Acad Sci . 1975 Feb 21;248:345-64. doi: 10.1111/j.1749-6632.1975.tb34197.x.
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Quantification of Polyacrylamide Gel Electrophoresis for Analysis of Whey Proteins - ScienceDirect
Polyacrylamide gel electrophoresis of whey proteins has been quantified by standardization of the separation and staining procedure. During each electrophoresis experiment, a standard solution of whey proteins was separated and stained under the
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Quantitative electrophoresis in polyacrylamide gels of 2–40% - ScienceDirect
Gels which contain too much bisacrylamide have a milky-opaque color. Thus, a milky gel is a good indication that excessive amounts of bisacrylamide are present. A 5 % gel containing 25o mg % of bisacrylamide shows a faint tinge of milky color. A
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Staining and quantification of proteins separated by polyacrylamide gel electrophoresis - ScienceDirect
Polyacrylamide gel electrophoresis (PAGE) and staining of proteins is relatively easy to perform, but quantification of separated proteins is often necessary. It is quite easy to measure stained protein bands with a densitometer, however, it is
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Quantification of proteins in polyacrylamide gels - Amino Acids & Proteins | Laboratory Methodology
Quantification of proteins in polyacrylamide gels. The SDS-polyacrylamide gel electrophoresis (SDS-PAGE) is undoubtedly a versatile technique for the characterization of proteins both quantitatively and qualitatively. It is an easy and quick
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Staining and quantification of proteins separated by polyacrylamide gel electrophoresis - ScienceDirect
The present review concentrates on techniques for the staining and quantification of proteins separated by polyacrylamide gel electrophoresis. Staining with organic dyes has been used for approximately thirty years; the silver staining technique
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Staining and quantification of proteins separated by polyacrylamide gel electrophoresis.
Staining and quantification of proteins separated by polyacrylamide gel electrophoresis. Syrový I(1), Hodný Z. Author information: (1)Institute of Physiology, Czechoslovak Academy of Sciences, Prague. The present review concentrates on
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Cellulose Acetate and Polyacrylamide Gel Electrophoresis for Quantification of Milk Protein Fractions - ScienceDirect
Cellulose acetate electrophoresis and PAGE techniques were compared for fractionation and quantification of milk proteins. Protein bands were stained with Ponceau-S and aniline blue black in the cellulose acetate electrophoresis and PAGE, respec
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Rapid Separation and Quantification of Major Caseins and Whey Proteins of Bovine Milk by Polyacrylamide Gel Electrophoresis - ScienceDirect
A rapid polyacrylamide gel electrophoretic method was developed for separating and quantifying major proteins in casein and whey protein fractions of bovine milk. For casein separation, best results were achieved by an 8% polyacrylamide gel cont
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Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue‐native polyacrylamide gel electrophoresis
Blue native‐polyacrylamide gel electrophoresis (BN‐PAGE) The supernatant enriched with mitochondrial complexes was supplemented with a 5% (w/v) stock solution of Serva Blue G in solubilization medium to a final ratio of 1:4 (w:w) of dye to
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Chip-based gel electrophoresis method for the quantification of half-antibody species in IgG4 and their by- and degradation products
Chip-based gel electrophoresis method for the quantification of half-antibody species in IgG4 and their by- and degradation products Anal Biochem. 2004 Nov 1;334(1):81-8. doi: 10.1016/j.ab.2004.07.002. Authors
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Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue-native polyacrylamide gel electrophoresis
Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue-native polyacrylamide gel electrophoresis Plant J . 2005 Dec;44(5):893-901. doi: 10.1111/j.1365-313X.2005.02577.x.
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Chemical quantification of melanophore stimulating substances using polyacrylamide gel disc electrophoresis.
Chemical quantification of melanophore stimulating substances using polyacrylamide gel disc electrophoresis. Preslock JP, Brinkley HJ. PMID: 5435309 [PubMed - indexed for MEDLINE] MeSH Terms Acrylates Adrenal Glands/metabolism Animals Anura Gels
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Determination of sulfhydryl groups and disulfide bonds in a protein by polyacrylamide gel electrophoresis
A general method by polyacrylamide gel electrophoresis for the determination of sulfhydryls and disulfides in a protein was developed. The method included a two-step alkylation procedure: the first step consisted of alkylation of the sulfhydryl
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SDS-PAGE, Sodium Dodecyl Sulfate–PolyAcrylamide Gel Electrophoresis–Animation
I make animations in biology with PowerPoint, this animation video is about DS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, which is an a...
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Essential problems in quantification of proteins following colloidal staining with coomassie brilliant blue dyes in polyacrylamide gels, and their
Quantitative determination of stained proteins following polyacrylamide gel electrophoresis (PAGE) is of increasing interest especially since computer-aided densitometers have become available as well as recipes for sensitive and background-free
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TAE and TBE Running Buffers Recipe & Video
TAE buffer has been utilized in agarose gel electrophoresis of RNA. 3,4 A study of free DNA solution mobility in TAE at various buffer concentrations, in the presence and absence of added NaCl, has been reported. 5 The use of TAE buffer in a
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Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue‐native polyacrylamide gel electrophoresis
Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue‐native polyacrylamide gel electrophoresis Mohammed Sabar Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1
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Vertical Capillary Machine Of Gel Hb Electrophoresis - Buy Hb Electrophoresis,Gel Hb Electrophoresis,Electrophoresis Machine Product
Vertical Capillary Machine Of Gel Hb Electrophoresis , Vertical Capillary Machine Of Gel Hb Electrophoresis,Hb Electrophoresis,Gel Hb Electrophoresis,Electrophoresis Machine from Other Lab Supplies Supplier or
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A novel gel electrophoresis technique for rap | EurekAlert!
BAC-DROP, our novel electrophoresis technology, uses a dissolvable form of polyacrylamide gel, which allows sample pretreatment to be completed in about 5 hours.
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Apolipoprotein A-IV: a protein occurring in human mesenteric lymph chylomicrons and free in plasma. Isolation and quantification.
Isolation and quantification. Utermann G, Beisiegel U. 1. Human mesenteric lymph chylomicrons were isolated from chylous ascites fluid by ultra-centrifugation and agarose/gel chromatography and their apoprotein composition was analysed by
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Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning.
Additionally, semi-quantitative polyacrylamide gel electrophoresis revealed a ~ 10-fold signal-to-noise ratio of LASSO capture in a simplified system. CONCLUSIONS: These results suggest that LASSO probe coverage for target sequences is more
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In-gel stable isotope labeling for relative quantification using mass spectrometry.
In-gel stable isotope labeling for relative quantification using mass spectrometry. Asara JM(1), Zhang X, Zheng B, Maroney LA, Christofk HR, Wu N, Cantley LC. Author information: (1)Division of Signal Transduction, Beth Israel Deaconess Medical
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Polyacrylamide gel electrophoresis
Polyacrylamide gel electrophoresis is a powerful tool used to analyze RNA samples. When polyacrylamide gel is denatured after electrophoresis, it provides information on the sample composition of the RNA species. 5NO) by nitrile hydratase.
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Method Development for an Easy and Direct Quantitation of Protein Adsorption by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS
Method Development for an Easy and Direct Quantitation of Protein Adsorption by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDSPAGE) by Man Chung Lee B.Sc, University of British Columbia, 2011 Thesis Submitted in Partial
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Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor.
Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor. Saso L(1), Leone MG, Sorrentino C, Giacomelli S, Silvestrini B, Grima J, Li JC, Samy E, Mruk D, Cheng CY.
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A multidimensional differential proteomic platform using dual-phase ion-exchange chromatography-polyacrylamide gel electrophoresis/reversed-phase
Demonstrated using a neuroproteomic model, CAX-PAGE revealed an increased number of differential proteins, 137, compared with 82 found by 2D difference gel electrophoresis. When combined with RPLC-MSMS for protein identification, an additional q
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Unit 2: Lecture 3: Nucleic Acid Detection/Quantification Flashcards | Quizlet
Start studying Unit 2: Lecture 3: Nucleic Acid Detection/Quantification. Learn vocabulary, terms, and more with flashcards, games, and other study tools. • Gel electrophoresis is the movement of a charged molecule in a matrix by an electric
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